Biogenic Synthesis of Nano-Silver and Their Anti-Microbial Efficacy

Rai Dhirendra Prasad1

Saurabh R. Prasad2

Om Prakash Shrivastav3

A.R. Kanthe4

S.R. Waghmare5

Viquar S. Shaikh6

Kailas M. Doke6

Neeraj R. Prasad4,5,Email

Gulam M.Nazeruddin6

Yaseen I. Shaikh6,Email

1Bihar Veterinary College, Patna - 800014, India
2DKTE Textile and Engineering Institute, Ichalkaranji, 416115, India
3Indian Agricultural Research Institute, Pusa- 848125, Bihar, India
4Jaysingpur College, Jaysingpur -415101 India
5Shivaji University, Kolhapur-416004, India
6Department of Chemistry Abeda Inamdar Senior College, Pune -411001, India



Rapid synthesis of silver nanoparticles through an economically feasible green chemistry approach is highly desirable. In this study, we have developed a novel method to synthesize silver nanoparticles using an aqueous extract of Zingiber officinale rhizome and silver salt solution (AgNO3) without the need for any surfactant or external energy. This experimentation produced physiologically stable, bio-compatible AgNPs which may be used for targeted drug delivery in veterinary medicine that could offers enhanced therapeutic efficacy with minimal side effects. In-vitro bio-synthesis of metal nanoparticles using extracts of plants like Azadirachta indica (Neem), and Papaver somniferum (Opium) has been successfully carried out by earlier researchers. In this article AgNPs formation using Zingiber officinale has been thoroughly discussed. It is well known that on treating the metallic salt solution with some plant extracts, a rapid reduction occurs leading to the formation of highly stable metal nanoparticles. i.e. reaction time happened to be 1 to 2 hours as compared to 2-4 days required by microorganisms. The end products of synthesis were analyzed by various characterization techniques to reveal their morphology, chemical composition, and antimicrobial activity. The synthesized nanomaterials shows potential activity against gram positive bacterium Bacilllus subtilus NCIM 2635. The hole size is 8mm and zone of inhibition is found to be 15mm. 

Biogenic Synthesis of Nano-Silver and Their Anti-Microbial Efficacy