Partial Characterization and Combination Therapy with a Pectic Polysaccharide from Semi-ripe Bael (Aegle marmelos) Fruit in a Murine Model of Visceral Leishmaniasis

Gora Das1,2,Email

Asish Kumar Sen2

1Haldia Institute of Technology, Haldia 721657, West Bengal, India
2Indian Institute of Chemical Biology,  Kolkata 700032 West Bengal, India

Abstract

The hot water soluble extract from semi-ripe Bael (Aegle marmelos) fruit pulp was fractionated by anion exchange chromatography. The major fraction (HWSPP) was found to consist of arabinose, galactose, galacturonic acid with traces of rhamnose. Methylation analysis of HWSPP showed the presence of (1→2,4) Rhap, (1→ 3,5)-Araf, (1→5)-Araf, t-Araf, t-Galp, (1→3,6) Galp, and (1→4) Galp.  HWSPP reduced the splenic and liver parasite burdens by 80% in Leishmania donovani infected BALB/c mice, where as sodium antimony gluconate (SAG) reduced the burdens approximately 40%. When infected animals received HWSPP plus SAG, there were approximately 95% clearance of parasites from the spleens and the livers, suggesting that HWSPP, a naturally derived pectic polysaccharide has potential as a new agent for sole or conjunctive therapy for leishmaniasis.

Partial Characterization and Combination Therapy with a Pectic Polysaccharide from Semi-ripe Bael (Aegle marmelos) Fruit in a Murine Model of Visceral Leishmaniasis