Studies on the Antidiarrheal Activity and Antimicrobial Activity of Aegle Marmelos Dried Fruit Pulp: Validating its Traditional Usage

Gora Das,1,3*Email

Alok Ghosh 2

Asish Kumar Sen3

1School of Applied Science and Humanities, Haldia Institute of Technology, Haldia 721657, India
2Department of Biochemistry, University of Calcutta, Kolkata 700073, India
3Department of Chemistry, Indian Institute of Chemical Biology, Kolkata 700032, India

Abstract

Aegle marmelos despite its traditional usage as an antidiarrhoeal activity there is limited information regarding its mode of action in infectious forms of diarrhea. Hence we have studied the hot water soluble pectic polysaccharide (HWSPP) isolated from aqueous extract of semi-ripe fruit pulp of A.marmelos for its antimicrobial activity and antidiarrhoeal activity. We have studied in vitro using pathogenic bacterial strains like Escherichia coli O114, Vibrio cholerae Inaba 569B, Entamoeba histolytica HM1:IMSS. At a concentration of 500 µg/ml HWSPP showed nearly 32% anti-vibriocidal activity w.r.t. control after 48h. Ciprofloxacin under similar experimental condition showed 33% anti-vibriocidal activity at a dose of 500 µg/ml w.r.t. control after 48h. After 48h HWSPP (500 µg/ml) showed 18.7% anti-E. coli activity w.r.t. control, whereas, under the same experimental condition the antibiotic ciprofloxacin (500 µg/ml) showed 32% inhibition. HWSPP (200 µg/ml) showed moderate anti-amoeba activity (40%) against Entamoeba histolytica (HM1:IMSS). Under similar experimental condition the reference compound Caryophyllene α-oxide (200 μg/ml) showed 47% anti-Entamoeba histolytica activity. The in vivo antidiarrhoeal activity of the HWSPP in mice showed dose dependent activity where, significant reduction of defaecation has been observed at a dose of 5 mg/kg body weight with respect to the control.
 

Studies on the Antidiarrheal Activity and Antimicrobial Activity of Aegle Marmelos Dried Fruit Pulp: Validating its Traditional Usage