Untangling the Effect of Surfactants as An Intermediate at Gold Nanoparticle-Antibiotic Interface for Enhanced Bactericidal Effect

Amritpal Kaur

Rajesh Kumar*Email

Department of Physics, Panjab University, Chandigarh, India, 160014 

Abstract

In the presence of different surfactants, gold nanoparticles were synthesized by a chemical method. Trisodium citrate, PVP, and Tween 20 were used to study their role as a binder between AuNPs and amikacin. UV-Visible spectroscopy, DLS, TEM, FTIR, and XRD have been used to investigate the interaction mechanism between amikacin and nanoparticle surface. The red and blue shifts in UV-Visible absorption peaks confirmed the formation of drug conjugates. The Z-average size before and after drug conjugation is another parameter that hints at the binding of a drug to the gold nanoparticle surface. Moreover, TEM confirmed that the morphology remains the same after the formation of drug conjugates. FTIR has been used to study the nature of the interaction between drug and nanoparticles. The results reveal that either it is the secondary amine or amide group that plays a role in the interaction between amikacin and the AuNPs surface through surfactants. A slight change in reflection lines in the XRD pattern also depicts the loading of amikacin on AuNPs. The bactericidal activity of synthesized samples has been tested against Staphylococcus aureus and Escherichia coli bacteria by calculating the zone of inhibition. An enhanced antibacterial effect has been observed against both bacteria.

Untangling the Effect of Surfactants as An Intermediate at Gold Nanoparticle-Antibiotic Interface for Enhanced Bactericidal Effect