Bioethanol production is gaining prominence as a viable biofuel option, addressing the growing need to reduce fossil fuel dependence. To explore cost-effective substrates, cheese whey emerges as a promising alternative for bioethanol production. This study assessed the organoleptic properties and taxonomic composition of three whey samples: "Merke diary" (technical), "Amiran" (commercial), and “Stella Alpina” (commercial). Highly promising strains from the whey samples: Kluyveromyces marxianus A1 and A2, and Saccharomyces cerevisiae M1 and M2 were identified by genetic analysis. The immobilisation of Kluyveromyces marxianus A1 cells with superior adsorption rates on k-carrageenan for a period of 96 hours yielded the highest bioethanol production. Thus, this research signifies the potential of whey as a valuable resource in bioethanol production, aligning with the global shift towards circular economy.