Applying an Optimization Technique for the Extraction of Antioxidant Components from Justicia adhatoda Leaves

Md. Anwar Hossain1,Email

Shammi Akhter2

Md. Hossain Sohrab2,Email

Farhana Afroz2

Mst. Nadira Begum3

Satyajit Roy Rony2

1Planning and Development Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka-1205, Bangladesh. 
2Pharmaceutical Science Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka-1205, Bangladesh. 
3Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka-1205, Bangladesh. 

Abstract

In this research work, the response surface approach was constructed on a central composite design (CCD) and was applied to control optimization for antioxidant elements extraction from Justicia adhatoda shrubberies. Two extraction parameters were assessed in 14 experimental trials as methanol: water (30% to 80%) and the extraction time (4 to 6 days). The quadratic polynomial equation of multiple regression analysis forecasts the extraction process's optimization. The maximum antioxidant extract (AE) was obtained at methanol (55%) and time (6.41 days) 6.48 g/100g on trial 09. The minimum radical scavenging activity (IC50) values were obtained at methanol (55%) and extraction time of 3.59 days on trial 08. It was found that the AE yields and radical scavenging activity (IC50) of Justicia adhatoda leaves could be optimized at 6.62 g/100g and 1.66 μg/mL at the optimum condition at a time of 6.41 days and solvent concentration of 76.01% with overall desirability 0.96. According to the response optimizer method, the conditions that maximize the yield and minimize the DPPH are within the established restrictions. The well-fitted actual values to predicted values validate the regression model acceptability. Antioxidant properties of Justicia adhatoda leaves using response surface methodology (RSM) confirm this technique's usefulness.

Applying an Optimization Technique for the Extraction of Antioxidant Components from Justicia adhatoda Leaves