Effects of Citrus aurantifolia Fruit Juice on Selected Pathogens of Public Health Importance

Sylvester Chibueze IzahEmail

Tamaraukepreye Catherine Odubo

Department of Microbiology, Faculty of Science, Bayelsa Medical University, PMB 178, Yenagoa, Bayelsa State, Nigeria.

Abstract

In this study, the effects of storage temperature and time condition on the antimicrobial properties of fruit juice of Citrus aurantifolia against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus, and Salmonella species was evaluated. Agar well diffusion method was adopted. The in situ pH, conductivity, salinity and total dissolved solid characteristics of the fruit juice of Citrus aurantifolia stored at different temperature conditions were 2.52  to 2.74, 5.06  to 5.85mS/cm, 2.58  to 2.95ppt, and 3.58  to 4.29ppt respectively. Under different temperature storage conditions and time intervals, the fruit juice of Citrus aurantifolia displayed variable efficacy against the selected bacterial species, thereby exhibiting bacteriostatic and bactericidal activities. The Minimum inhibitory concentrations (MIC) and Minimum Bactericidal Concentration (MBC) determination for all the test organisms at different temperature storage conditions and time intervals ranged from 12.50 to 50.00%. However, there was a statistical deviation (p<0.05) across the different temperature storage conditions and time intervals for the in situ characteristics, MIC, and MBC. The acidity of Citrus aurantifolia juice inhibited the growth of many bacteria and may be used as a natural alternative to chemical antimicrobials to prevent food poisoning and to keep food fresh without the health risks associated with synthetic chemicals.

Effects of Citrus aurantifolia Fruit Juice on Selected Pathogens of Public Health Importance