Nanoparticles and Bacterial Interaction of Host-Pathogens and the Detection Enhancement of Biomolecules by Fluorescence Raman Spectroscopic Investigation

G. Satpathy1, 2

G. K. Chandra3

K. Elayaraja4

D. R. Mahapatra2

A. Subramania5

Zhanhu Guo6,Email

Siva Umapathy7,8

E. Manikandan1,9,10,Email

1Central Research Laboratory, Dept. of Biotechnology, Sree Balaji Medical College & Hospital (SBMCH), Bharath Institute for Higher Education & Research (BIHER), Chennai-600073, Tamil Nadu, India
2Laboratory of Integrative Multiscale Engineering Materials and Systems, Dept. of Aerospace Engineering, Indian Institute of Science, Bangalore-560012, India
3Dept. of Physics, National Institute of Tehnology (NIT), Calicut-673601, Kerala, India
4Dept. of Materials Science and Engineering, Advanced Materials Processing and Analysis Center, University of Central Florida, Orlando, FL, USA
5Centre for Nanoscience & Technology, Pondicherry University, Puducherry, India
6Integrated Composites Laboratory (ICL), Dept. of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, USA 
7Dept. of Inorganic and Physical Chemistry, Inian Institute of Science (IISc), Bangalore-560012, India
8Indian Institute of Science Education and Research (IISER), Bhopal, MP, 462066, India
9UNESCO-UNISA Africa Chair in Nanoscience/Nanotechnology, College of Graduate Studies, University of South Africa, Muckleneuk Ridge, P. O. Box 392, Pretoria, South Africa
10Dept. of Physics, TUCAS Campus, Thennangur-604408, Thiruvalluvar University, Vellore, India

Abstract

In this investigation, metal nanoparticles (NPs) have been used as antibiotics to inhibit the growth of bacteria. Here, we report an antibacterial study of ZnO-NPs, Co-NPs, and Au-NPs towards the approach for detecting the pathogenic strain of Escherichia coli (E. coli) cells. The nanoparticle (NP) acts as the detector probe for both live and dead pathogenic E. coli cells. The aim is to investigate the antibacterial bio-activities of metal NPs and their mode of action against pathogenic bacteria on the whole cell. Antibacterial activities of NPs were assessed by the agar diffusion method quantitatively and the quality and quantity of E.coli cells extraction in the presence of nanoparticles (ZnO-NPs, Au-NPs, Co-NPs). The optimized concentration kills the pathogenic E. coli cells and hence shows the enrichment of cells. Results indicate the highly efficient nanoparticles inhibit the growth of such pathogenic microorganisms. Nanoparticle metal oxides signify a novel course of vital materials that are progressively being advanced for use in research and health-related applications. At the same time, we use Raman spectroscopy and fluorescence microscopic images for a better understanding of the effect of NPs on bacterial cells.

Nanoparticles and Bacterial Interaction of Host-Pathogens and the Detection Enhancement of Biomolecules by Fluorescence Raman Spectroscopic Investigation