In this work, relationship involving servant leadership, ethical climate employees of manufacturing industries and educational institutions is investigated and established. The questionnaires were included to collect the data from full-time employees of managers, engineers, and officers, teaching faculty of their organization. In the servant leadership questionnaire, there are 23 items, five dimensions, and in the ethical climate questionnaire, there are 35 items and nine dimensions. The final research survey was carried out on a convenience sample of 307 from manufacturing industries and 324 samples from educational institution using a non-probability sampling strategy. The confirmatory-factor analysis, reliability and adequacy of the sample are tested using Statistical package for the social sciences (SPSS) version 22. The independent sample test hypothesis proves that servant leadership and the ethical climate of both manufacturing industries and educational institutions employees are analogous and the regression analysis hypothesis proves that the existence of a solid association between servant leadership and ethical climate employees of manufacturing industries and educational institutions. The conducted study will be helpful to manufacturing industries and educational institutions to frame the policies based on servant leadership and ethical climate to motivate the employees to strive for organization excellence.