This paper reports the caliber of surfactant coated gold nanoparticles (AuNPs) as a carrier for antibiotic and investigate the possible interaction mechanism between AuNPs and antibiotic through different surfactants. Further, the antibacterial efficacy of the drug-AuNPs complex has been evaluated by agar well diffusion method. The AuNPs used in this work were synthesized by chemical reaction method using gold chloride (AuCl3) as a precursor and sodium borohydride (NaBH4) as a reducing agent. Further, the surfactant coated AuNPs were functionalized with a glycopeptide antibiotic named as vancomycin. The formation of the drug-AuNPs complex was confirmed by UV-Visible absorption spectroscopy. Fourier transform infrared spectroscopy (FTIR) was used to investigate the nature of the interaction between drug and AuNPs. The increase in the hydrodynamic size of drug coated AuNPs in comparison to bare AuNPs also attributed to the formation of the drug-AuNPs conjugate. Transmission electron microscopy (TEM) was used to investigate the morphology of synthesized drug-AuNPs conjugate. X-ray diffraction (XRD) was carried out to study the crystalline structure of AuNPs and drug coated AuNPs. Antibacterial activity of synthesized samples has been tested against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strain.