The interactions in aqueous solutions of alanine, arginine, glutamine and histidine (0.02-0.20) mol∙kg−1 in different concentrations of aqueous drug (0.001, 0.005, and 0.010) mol∙kg−1 have been studied over temperature range 293.15 to 313.15 K with an interval of 5K by using density, speed of sound and viscosity measurements. The density and speed of sound data have been used to calculate various parameters viz. apparent molar volume, partial molar volume apparent molar isentropic compressibility, partial molar isentropic compression etc. The density and speed of sound data have been used to calculate various parameters. These interactions have been interpreted in terms of the presence of different kinds of intermolecular interactions in (amikacin sulphate + water + amino acid) system i.e. ion-ion and ion-hydrophilic interactions, dominate over the ion-hydrophobic interactions. Moreover, viscosity data have been analyzed by employing Jones-Dole equation and information on solute-solute and solute-solvent interactions have been extracted. Also, transition state theory has been applied to evaluate thermodynamic activation parameters and more disordered transition state due to rupture and distortion of intermolecular forces in the system have been observed. For the studied ternary systems, the absorption spectra have also been recorded. Moreover, the amino acids act as efficient structure maker in amikacin sulphate solution.