Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties

Abeer Ahmed Qaed Ahmed,1

Ruizhu Zheng,1

Ahmed M. E. Abdalla,3*Email

Bianza Moise Bakadia,1

Fuyu Qi,1

Lin Xiao,2*Email

Omar Mohammad Atta,1

Lin Mao1 

Guang Yang1*Email

1 Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.

2 School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, 510006, China

3 Department of Biochemistry, College of Applied Science, University of Bahri, Khartoum, 1660/11111 Sudan

Abstract

Gram-negative bacteria release outer membrane vesicles (OMVs) with great potential in a variety of biomedical applications. The study aimed to investigate nutritional growth supplements (HBS, FBS, β-Cyclodextrin, or Cholesterol) and their impact on H. pylori’s OMVs biological properties. SDS-page showed a difference in protein components between all OMVs populations. Nuclear morphology for cells treated with 40 μg/mL of ExHp-CD displayed no change in nuclear morphology, while cells treated with ExHp-HBS, ExHp-FBS, and ExHp-Chlos showed chromatin condensation and nuclear fragmentation. CCK-8, LDH, and WST-1 assays showed no adverse effect for ExHp-CD in all used concentrations, while treated cells were negatively affected under higher concentrations for ExHp-HBS and ExHp-FBS (40 and 50 μg/mL) or in almost all the used concentrations for ExHp-Chlos. Obtained OMVs populations were phagocyted by macrophage RAW 264.7 cells leading to activate macrophages and produce cytokines. IL-10 and IL-4 were significantly (p<0.05) increased after stimulation with all obtained OMVs, while levels of IFN-γ and IL-12 were not significantly increased. All OMVs populations increased CD206 ratio over CD86 ratio, demonstrating M2-phenotype polarization. Current findings revealed that growth supplements influenced OMVs biological properties while inducing Th2-immune response. This knowledge could determine which type of OMVs population for the required biomedical application.

Heterogeneous Populations of Outer Membrane Vesicles Released from Helicobacter Pylori SS1 with Distinct Biological Properties