Mechanochemical Synthesis of Hydrophilic Sulfur Nanoparticles in Aqueous Surfactant Solutions and Their Antibacterial Activity and Acute Toxicity in Mice

Seitzhan Turganbay1,2,Email

Saule Aidarova1

Saltanat Kumargaliyeva3

Dias Argimbayev1

Aitugan Sabitov4

Zhanar Iskakbayeva2

Marina Lyu2

Naiyla Ibragimova2

Gulsinay Turganbay5

1Kazakh-British Technical University, "One Belt, One Road" Petroleum Engineering, Almaty ,050000, Republic of Kazakhstan.
2JSC Scientific Center for Anti-Infectious Drugs,  Laboratory of New substances and materials, Almaty 050060, Republic of Kazakhstan.
3Al-Farabi Kazakh National University. Department of Analytical, Colloidal Chemistry and Technology of Rare Elements 050000, Almaty, Kazakhstan.
4Combustion Problems Institute, Almaty, Nanobiotechnology Laboratory, 05000, Republic of Kazakhstan.
5Kazakh National Agrarian University, Agronomy, breeding and biotechnology, Almaty 050000, Republic of Kazakhstan.

Abstract

Sulfur is an abundantly available biologically active element and has been used for a long time as a bactericidal and fungicidal drug in both medical, veterinary and in agricultural practices. Synthesized hydrophilic sulfur nanoparticles can potentially allow the delivery of high concentrations of sulfur to infections. In the present study, a quick and environmentally friendly approach has been developed for the synthesis of hydrophilic sulfur nanoparticles with antibacterial activity. hydrophilic sulfur nanoparticles (SNPs) were prepared by modifying the surface of elemental sulfur by various water-soluble surfactants including hexadecyltrimethylammonium bromide (CTAB), sodium dodecyl benzene sulfonate (SDBS) and Triton X-100 (TX-100). The hydrophilic SNPs were characterized by UV–visible spectrophotometer, X-ray diffraction (XRD), and thermogravimetric analysis (TG/DSC), scanning electron microscope (SEM), the hydrophilic SNPs showed an almost spherical shape with an average size in the range of 150-200 nm. The antibacterial activity of the hydrophilic sulfur nanoparticles was tested using against Gram-positive (S. aureus and E. faecium) and Gram-negative (Escherichia coli and P. aeruginosa) bacteria. As a result, that hydrophilic SNPs exhibited significant antimicrobial activity against Gram-positive bacteria, i.e., S. aureus and E. faecium. The acute toxicity of the hydrophilic SNPs was studied at a single oral administration of solutions of hydrophilic SNPs, as a result of the study of hydrophilic SNPs, it was determined as a non-toxic substance. The received products can be considered as perspective forms of application in agriculture and medicine.

Mechanochemical Synthesis of Hydrophilic Sulfur Nanoparticles in Aqueous Surfactant Solutions and Their Antibacterial Activity and Acute Toxicity in Mice